Incorporation of /sup 35/S-sulfate and /sup 3/H-glucosamine into heparan and chondroitin sulfates during the cell cycle of B16-F10 cells

Journal Article · Tue May 01 00:00:00 EDT 1984 · Cytometry (Baltimore); (United States)

OSTI ID:6472695

Blair, O C; Sartorelli, A C

Changes in glycosaminoglycan composition occurring during the cell cycle were determined in B16-F10 cells sorted flow cytometrically with respect to DNA content. Incorporation of /sup 35/S-sulfate into heparan sulfate and chondroitin sulfate of unsorted and G1,S, and G2 +M sorted cells was determined following chondroitinase ABC or nitrous acid treatment; the incorporation into surface material was measured as the difference between the radioactivity of control and trypsin-treated cells. Incorporation of /sup 35/S-sulfate and /sup 3/H-glucosamine into cetyl pyridinium chloride (CPC)-precipitable material was characterized before and after chondroitinase or nitrous acid treatment by Sephadex G50 chromatography. Long-term (48 h) and short-term (1 h) labeling studies demonstrate that (a) the amount of total cellular chondroitin sulfate is greater than that of heparan sulfate, with larger amounts of unsulfated heparan than chondroitin being present; (b) the rate of turnover of heparan sulfate is greater than that of chondroitin sulfate; (c) greatest short-term incorporation of 3H-glucosamine into CPC-precipitable material occurs during S phase; and (d) the rate of turnover of both heparan sulfate and chondroitin sulfate is decreased in S phase relative to G1 and G2 + M.

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 6472695
• Journal Information: Cytometry (Baltimore); (United States), Vol. 5:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSAMINE
BIOCHEMISTRY
CELL CYCLE
CELL FLOW SYSTEMS
CHONDROITIN
MELANOMAS
SULFATES
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques