Differential stability of c-myc mRNAS in a cell-free system
The authors have developed a simple cell-free system for studying the stability of different mRNAs in vitro. They demonstrate that the threefold greater stability in vivo of truncated c-myc mRNA (lacking exon 1) compared with that of full-length c-myc mRNA is maintained in our in vitro system. Chimeric mRNAs in which the first exon of c-myc was fused to immunoglobulin C ..cap alpha.. heavy chain of glyceraldehyde-3-phosphate dehydrogenase mRNAs were not rapidly degraded, demonstrating that c-myc exon 1 alone is not sufficient to tag mRNAs for rapid degradation. Competition experiments show that full-length c-myc mRNA is specifically recognized by a factor(s) responsible for its rapid degradation. This system will allow further characterization and purification of these factors.
- Research Organization:
- Dept. of Biological Chemistry and the Molecular Biology Institute, Univ. of California, Los Angeles, CA (US)
- OSTI ID:
- 6470856
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MESSENGER-RNA
STABILITY
ONCOGENES
DNA-CLONING
EXPERIMENTAL DATA
HYBRIDIZATION
IMMUNOGLOBULINS
IN VITRO
NUCLEOTIDE DEHYDROGENASES
RNA PROCESSING
CLONING
DATA
DNA HYBRIDIZATION
ENZYMES
GENES
GLOBULINS
INFORMATION
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RNA
550200* - Biochemistry