Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1

Koh, Sung-Cheol; Bowman, J P; Sayler, G S

Title: Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1

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Abstract

Soluble methane monooxygenase (sMMO), present in certain methanotrophic bacteria, can oxidize a wide range of carbon substrates including halogenated aliphatic compounds such as trichloroethylene (TCE), a significant ground water pollutant. This study reports the existence of sMMO activity in a type I methanotroph (Methylosinus trichosporium 68-1) isolated from a TCE-contaminated aquifer. The sMMO of 68-1 was compared with that of Methylosinus trichosporium OB3b in terms of TCE degradation kinetics and genetic homology. This study shows that a typical type I methanotrophy (68-1) has the ability to produce sMMO and rapidly degrade TCE, producing a relatively high biomass under the apparent nonoptimal state of copper limitation. As a result, this strain may have considerable bioremediation potential. Further studies will be necessary to determine exactly how the 68-1 sMMO differs from the sMMOs of OB3b and Methylococcus capsulatus both in terms of DNA and protein sequences. 48 refs., 4 figs., 2 tabs.

Authors:

Koh, Sung-Cheol; Bowman, J P; Sayler, G S ^[1]

Univ. of Tennessee, Knoxville (United States)

Publication Date:: Thu Apr 01 00:00:00 EST 1993

OSTI Identifier:: 6466387

DOE Contract Number:: AC05-84OR21400

Resource Type:: Journal Article

Journal Name:: Applied and Environmental Microbiology; (United States)

Additional Journal Information:: Journal Volume: 59:4; Journal ID: ISSN 0099-2240

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GROUND WATER; CONTAMINATION; HALOGENATED ALIPHATIC HYDROCARBONS; BIODEGRADATION; METHANOTROPHIC BACTERIA; DECONTAMINATION; AQUIFERS; BIOMASS; CARBON; COPPER; DNA; ENZYMES; KINETICS; POLLUTANTS; SUBSTRATES; BACTERIA; CHEMICAL REACTIONS; CLEANING; DECOMPOSITION; ELEMENTS; ENERGY SOURCES; HYDROGEN COMPOUNDS; METALS; MICROORGANISMS; NONMETALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXYGEN COMPOUNDS; PROTEINS; RENEWABLE ENERGY SOURCES; TRANSITION ELEMENTS; WATER; 560300* - Chemicals Metabolism & Toxicology

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                    Koh, Sung-Cheol, Bowman, J P, and Sayler, G S. Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1.  United States: N. p., 1993. 
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                    Koh, Sung-Cheol, Bowman, J P, & Sayler, G S. Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1.  United States.

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                    Koh, Sung-Cheol, Bowman, J P, and Sayler, G S. 1993.  
        "Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1".  United States.

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@article{osti_6466387,

  title        = {Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1},

  author       = {Koh, Sung-Cheol and Bowman, J P and Sayler, G S},

  abstractNote = {Soluble methane monooxygenase (sMMO), present in certain methanotrophic bacteria, can oxidize a wide range of carbon substrates including halogenated aliphatic compounds such as trichloroethylene (TCE), a significant ground water pollutant. This study reports the existence of sMMO activity in a type I methanotroph (Methylosinus trichosporium 68-1) isolated from a TCE-contaminated aquifer. The sMMO of 68-1 was compared with that of Methylosinus trichosporium OB3b in terms of TCE degradation kinetics and genetic homology. This study shows that a typical type I methanotrophy (68-1) has the ability to produce sMMO and rapidly degrade TCE, producing a relatively high biomass under the apparent nonoptimal state of copper limitation. As a result, this strain may have considerable bioremediation potential. Further studies will be necessary to determine exactly how the 68-1 sMMO differs from the sMMOs of OB3b and Methylococcus capsulatus both in terms of DNA and protein sequences. 48 refs., 4 figs., 2 tabs.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6466387},
  journal      = {Applied and Environmental Microbiology; (United States)},

  issn         = {0099-2240},

  number       = ,

  volume       = 59:4,

  place        = {United States},

  year         = {Thu Apr 01 00:00:00 EST 1993},

  month        = {Thu Apr 01 00:00:00 EST 1993}

}

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