Cell killing and mutation to 6-thioguanine resistance after exposure to tritiated amino acids and tritiated thymidine in cultured mammalian cells (L5178Y)

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577009· OSTI ID:6465853

Furuno-Fukushi, I; Ueno, A M; Matsudaira, H

Cell killing and mutation to 6-thioguanine resistance were studied in growing mouse leukemia cells in culture after exposure to tritiated amino acids and tritiated thymidine. These effects varied widely among the tritiated compounds tested, being greatest for tritiated thymidine followed by tritiated arginine and tritiated lysine, in that order, for a given concentration of /sup 3/H expressed in kBq/ml of /sup 3/H in the medium. The differences between each tritiated amino acid disappeared almost totally when the effects were compared on the basis of the absorbed dose to the cells. The effects of tritiated thymidine, however, remained more than twofold greater compared to other tritiated compounds. These results indicate the importance of determining the absorbed dose for assessment of the radiotoxicity of tritiated organic compounds. For an exceptional case (tritiated thymidine), contribution of a mechanism(s) other than beta irradiation should also be taken into account.

• Research Organization: National Institute of Radiological Sciences, Chiba-shi, Japan
• OSTI ID: 6465853
• Journal Information: Radiat. Res.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
TRITIUM COMPOUNDS
RADIATION INJURIES
TUMOR CELLS
CELL KILLING
MUTATIONS
AMINO ACIDS
CELL CULTURES
GUANINE
LABELLED COMPOUNDS
LEUKEMIA
MICE
SURVIVAL TIME
THYMIDINE
TOXICITY
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
DISEASES
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
MAMMALS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATION EFFECTS
RIBOSIDES
RODENTS
VERTEBRATES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells