Characterization of the TPR-MET oncogene p65 and the MET protooncogene p140 protein-tyrosine kinases
The proteins encoded by the human TPR-MET oncogene (p65/sup tpr-met/) and the human MET protooncogene (p140/sup met/) have been identified. The p65/sup tpr-met/ and p140/sup met/, as well as a truncated TPR-MET product expression in Escherichia coli, p50/sup met/, are autophosphorylated in vitro on tyrosine residues. Using the immunocomplex kinase assay, p140/sup met/ activity was detected in various human tumor epithelial cell lines. In vivo, p65/sup tpr-met/ is phosphorylated on both serine and tyrosine residues, while p140/sup met/ is phosphorylated on serine and threonine. p140/sup met/ is labeled by cell-surface iodination procedures, suggesting that it is a receptor-like transmembrane protein-tyrosine kinase.
- Research Organization:
- National Cancer Institute, Frederick, MD (USA)
- OSTI ID:
- 6464215
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:1
- Country of Publication:
- United States
- Language:
- English
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ONCOGENES
MOLECULAR BIOLOGY
PROTEINS
AUTORADIOGRAPHY
ELECTROPHORESIS
EPITHELIUM
ESCHERICHIA COLI
IODINATION
IODINE 125
MAN
METHIONINE
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RECEPTORS
SERINE
SODIUM IODIDES
SOMATIC CELLS
SULFUR 35
TUMOR CELLS
TYROSINE
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
GENES
HALIDES
HALOGEN COMPOUNDS
HALOGENATION
HYDROXY ACIDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
RADIOISOTOPES
SODIUM COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRANSFERASES
VERTEBRATES
550401* - Genetics- Tracer Techniques