Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-190-42846· OSTI ID:6462997

Kaperonis, A A; Chien, S

Plasma /sup 125/I-albumin was used as a marker of extracellular dilution in order to study the effect of high-speed centrifugation on transmembrane water distribution in several types of human red cells, including normal (AA), hemoglobin variants (beta A, AS, SC, beta S, and SS), and those from patients with hereditary spherocytosis. SS and AA erythrocytes were also examined for changes in intracellular hemoglobin concentration of three different density fractions and with increasing duration of spin. The minimum force and duration of centrifugation required to impair water permeability were found to vary with the red cell type, the anticoagulant used (heparin or EDTA), the initial hematocrit of the sample centrifuged, as well as among the individual erythrocyte fractions within the same sample. When subjecting pathologic erythrocytes to high-speed centrifugation, the /sup 125/I-albumin dilution technique can be used to determine whether the centrifugation procedure has led to an artifactual red cell water loss and to correct for this when it does occur. An abnormal membrane susceptibility to mechanical stress was demonstrated in erythrocytes from patients with hereditary spherocytosis and several hemoglobinopathies.

• Research Organization: Columbia Univ., New York, NY (USA)
• OSTI ID: 6462997
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Vol. 190:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PERMEABILITY
CENTRIFUGATION
BIOLOGICAL EFFECTS
HEMIC DISEASES
PATHOGENESIS
WATER
MEMBRANE TRANSPORT
ALBUMINS
BLOOD COUNT
ERYTHROCYTES
IODINE 125
ISOTOPE DILUTION
MAN
THALASSEMIA
ANEMIAS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SYMPTOMS
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques