Phosphorus-31 nuclear magnetic resonance spectroscopic study of the canine pancreas: applications to acute alcoholic pancreatitis
The first nuclear magnetic resonance spectroscopic study of the canine pancreas is described. Both in-vivo, ex-vivo protocols and NMR observables are discussed. The stability of the ex-vivo preparation based on the NMR observables is established for at least four hours. The spectra obtained from the in-vivo and ex-vivo preparations exhibited similar metabolite ratios, further validating the model. Metabolite levels were unchanged by a 50% increase in perfusion rate. Only trace amounts of phosphocreatine were observed either in the intact gland or in extracts. Acute alcoholic pancreatitis was mimicked by free fatty acid infusion. Injury resulted in hyperamylasemia, edema (weight gain), increased hematocrit and perfusion pressure, and depressed levels of high energy phosphates.
- Research Organization:
- Johns Hopkins Univ., School of Medicine, Baltimore, MD (USA)
- OSTI ID:
- 6462908
- Journal Information:
- Adv. Alcohol Subst. Abuse; (United States), Vol. 7:3-4
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ETHANOL
BIOLOGICAL EFFECTS
METABOLIC DISEASES
PATHOGENESIS
PANCREAS
NMR SPECTRA
BIOLOGICAL MODELS
DOGS
METABOLITES
NUCLEAR MAGNETIC RESONANCE
PERFUSED ORGANS
PHOSPHOCREATINE
PHOSPHORUS 31
TRACER TECHNIQUES
ALCOHOLS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE GLANDS
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques
560300 - Chemicals Metabolism & Toxicology