Quiescent human peripheral blood lymphocytes do not contain a sizable amount of preexistent DNA single-strand breaks

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90085-2· OSTI ID:6461534

Boerrigter, M E; Mullaart, E; van der Schans, G P; Vijg, J

Sedimentation of nucleoids through neutral sucrose density gradients has shown that nucleoids isolated from phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes (PBL) sediment faster than nucleoids derived from quiescent lymphocytes, which was attributed to rejoining of DNA single-strand breaks (SSB) present in the resting cells. We isolated PBL from donors and determined the amount of SSB in nonradiolabeled, untreated resting and PHA-stimulated cells by applying the alkaline filter elution technique. Calibration was based on dose-dependent induction of SSB by /sup 60/Co-gamma-radiation. Quiescent cells did not contain a sizable amount of SSB. Mitogen-stimulated cells showed equally low amounts of SSB per cell. The present study indicates that the interpretation of the results obtained with the nucleoid sedimentation technique concerning the supposed rejoining of SSB in PHA-stimulated human lymphocytes is incorrect. Other, equally sensitive, techniques such as alkaline filter elution appear to be preferable for studies on DNA damage and repair.

• Research Organization: TNO Institute for Experimental Gerontology, Rijswijk (Netherlands)
• OSTI ID: 6461534
• Journal Information: Exp. Cell Res.; (United States), Vol. 180:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
STRAND BREAKS
LYMPHOCYTES
GENETIC RADIATION EFFECTS
RADIOINDUCTION
CALIBRATION
COBALT 60
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
MAN
MITOGENS
PHYTOHEMAGGLUTININ
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUCOPROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture