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Title: Solid-state ethanol fermentation by means of inert gas circulation

Journal Article · · Biotechnol. Bioeng.; (United States)

A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packed-bed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethanol production was set in by a form of parallel fermentation using a respiration-deficient mutant of Saccharomyces cerevisiae. Produced ethanol was simultaneously stripped by circulating inert gas and separated in a condenser. Average ethanol concentration in the condensate was over 200 g/L, and over 90% of produced ethanol was recovered from the packed bed during 15 or 16 days of stripping. The fermentation efficiency was about 80%, which was evaluated much higher than those of conventional solid-state fermentations. The residue had lesser volume and a higher solids content compared with the distillery wastewaters of conventional liquid-state fermentations. This means an advantage for the treatment and the effective conversion of the residue into fertilizers or animal feeds.

Research Organization:
National Research Institute of Brewing, Tokyo, Japan
OSTI ID:
6457613
Journal Information:
Biotechnol. Bioeng.; (United States), Vol. 27:9
Country of Publication:
United States
Language:
English