Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone
- Univ. of Kentucky, Lexington
Three groups of 28 1-month-old male Sprague-Dawley rats each were fed a basal vitamin E-deficient diet and supplemented with either 0, 11, or 110 ppM vitamin E for 38 days, and were then exposed to 0 or 0.1 ppM ozone continuously for 7 days. Following ozone exposure, the level of reduced glutathione (GSH) and activities of GSH peroxidase, GSH reductase, glucose-6-phosphate dehydrogenase (G-6-PD), and lactate dehydrogenase (LDH), but not of malic dehydrogenase, were significantly elevated in the lungs of rats fed the vitamin E deficient diet. The level of GSH and activities of GSH peroxidase and G-6-PD were also significantly increased in the lungs of the animal group fed the 11 ppM vitamin E diet, while none of the biochemical measurements made was significantly altered by ozone in the 110-ppM vitamin E diet fed rats. Scanning electron microscope examination revealed that five out of six rats on the vitamin E-deficient diet and four out of six from the 11-ppM vitamin E diet had detectable lesions following ozone exposure, as compared with only one of the six exposed animals from the 110-ppM vitamin E diet. The lesion was restricted to bronchiolar epithelium and alveoli immediately adjacent to the bronchiole-alveolar duct junction. None of the control animals had detectable lesions. The results suggest that exposure to ozone at 0.1-ppM level can produce detectable pulmonary damage, and that dietary vitamin E alters pulmonary susceptibility to ozone exposure.
- OSTI ID:
- 6453422
- Journal Information:
- Environ. Res.; (United States), Vol. 24:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
OZONE
TOXICITY
RESPIRATORY SYSTEM
SENSITIVITY
VITAMIN E
BIOLOGICAL EFFECTS
BIOCHEMISTRY
COMPARATIVE EVALUATIONS
DIET
ENZYME ACTIVITY
GLUTATHIONE
METABOLISM
MORPHOLOGICAL CHANGES
MORPHOLOGY
NUTRITIONAL DEFICIENCY
QUANTITY RATIO
RATS
TIME DEPENDENCE
ANIMALS
CHEMISTRY
DRUGS
MAMMALS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
VERTEBRATES
VITAMINS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550200 - Biochemistry