CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus Saimiri gene

Journal Article · Tue Jun 15 00:00:00 EDT 1993 · Journal of Immunology; (United States)

OSTI ID:6449158

Rouvier, E; Luciani, M F; Golstein, P ^[1]; Mattei, M G ^[2]; Denizot, F ^[3]

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1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille (France)
2. INSERM U242, Marseille (France)
3. Centre de Sequencage d'ADN, Marseille (France)

To detect novel molecules involved in immune functions, a subtracted cDNA library between closely related murine lymphoid cells was prepared using improved technology. Differential screening of this library yielded several clones with a very restricted tissue specificity, including one that was named CTLA-8. CTLA-8 transcripts could be detected only in T cell hybridoma clones related to the one used to prepare the library. Southern blots showed that the CTLA-8 gene was single copy in mice, rats, and humans. By radioactive in situ hybridization, the CTLA-8 gene was mapped at a single site on mouse chromosome 1A and human chromosome 2q31, in a known interspecific syntenic region. The CTLA-8 cDNA sequence indicated the presence, in the 3'-untranslated region of the mRNA, of AU-rich repeats previously found in the mRNA of various cytokines, growth factors, and oncogenes. The CTLA-8 cDNA contained an open reading frame encoding a putative protein of 150 amino acids. This protein was 57% homologous to the putative protein encoded by the ORF13 gene of herpesvirus Saimiri, a T lymphotropic virus. These findings are discussed in the context of other genes of this herpesvirus homologous to known immunologically active molecules. More generally, CTLA-8 may belong to the growing set of virus-captured functionally important cellular genes related to the immune system or to cell death and cell survival. 69 refs., 5 figs.

• OSTI ID: 6449158
• Journal Information: Journal of Immunology; (United States), Vol. 150:12; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
HUMAN CHROMOSOME 2
MAPPING
LYMPHOCYTES
VIRUSES
DNA HYBRIDIZATION
DNA-CLONING
GROWTH FACTORS
MESSENGER-RNA
ONCOGENES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
HUMAN CHROMOSOMES
HYBRIDIZATION
LEUKOCYTES
MATERIALS
MICROORGANISMS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics