Differential acylation in vitro with tetradecanoyl coenzyme A and tetradecanoic acid (+ATP) of three polypeptides shown to have induced synthesis in Photobacterium phosphoreum
Acylation of extracts of Photobacterium phosphoreum at different stages of growth with (/sup 3/H)tetradecanoic acid (+ATP) has shown that two polypeptides found in the fatty acid reductase complex, the fatty acid activating enzyme (50K) and the 34K polypeptide, were specifically labeled during induction of the luminescent system. An alternate method for in vitro acylation of polypeptides in the luminescence system was developed using tetradecanoyl-CoA. Both the 34K polypeptide and, to a lesser extent, the acyl-CoA reductase component (58K) in the complex, were acylated with (/sup 3/H)tetradecanoyl-CoA. In contrast, the fatty acid activating enzyme (50K) was not labeled. Labeling of both the 34K and 58K polypeptides with (/sup 3/H)tetradecanoyl-CoA as well as the acyl-CoA reductase activity in extracts paralleled the induction of luciferase during growth. Differential labeling of P. phosphoreum cells with (/sup 35/S)methionine before luminescence induction and with (/sup 3/H)methionine after the onset of luminescence followed by purification of luciferase and the polypeptides in the fatty acid reductase complex demonstrated that the ..cap alpha.. and ..beta.. subunits of luciferase and the 34K, 50K and 58K polypeptides of the complex had /sup 3/H//sup 35/S ratios at least 7-fold higher than the constitutive proteins. These results give evidence that the synthesis of the component polypeptides of the fatty acid reductase are induced during the development of bioluminescence and may be under the same control as luciferase. The experiments also showed that P. phosphoreum may have the highest content of luciferase of any luminescent bacterium, constituting approximately 20% of the total soluble protein in extracts.
- Research Organization:
- McGill Univ., Montreal, Quebec
- OSTI ID:
- 6444366
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 259:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHOTOSYNTHETIC BACTERIA
BIOLUMINESCENCE
POLYPEPTIDES
ACYLATION
BIOSYNTHESIS
ISOTOPE RATIO
LUCIFERASE
OXIDOREDUCTASES
SULFUR 35
TETRADECANOIC ACID
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LUMINESCENCE
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
550701* - Microbiology- Tracer Techniques