Involvement of a new enzyme, glyoxal oxidase, in extracellular H/sub 2/O/sub 2/ production by Phanerochaete chrysosporium
The importance of extracellular H/sub 2/O/sub 2/ in lignin degradation has become increasingly apparent with the recent discovery of H/sub 2/O/sub 2/-requiring ligninases produced by white-rot fungi. Here the authors describe a new H/sub 2/O/sub 2/-producing activity of Phanerochaete chrysosporium that involves extracellular oxidases able to use simple aldehyde, ..cap alpha..-hydroxycarbonyl, or..cap alpha..-dicarbonyl compounds as substrates. The activity is expressed during secondary metabolism, when the ligninases are also expressed. Analytical isoelectric focusing of the extracellular proteins, followed by activity staining, indicated that minor proteins with broad substrate specificities are responsible for the oxidase activity. Two of the oxidase substrates, glyoxal and methylglyoxal, were also identified, as their quinoxaline derivatives, in the culture fluid as secondary metabolites. The significance of these findings is discussed with respect to lignin degradation and other proposed systems for H/sub 2/O/sub 2/ production in P. chrysosporium.
- Research Organization:
- Dept. of Agriculture, Madison, WI
- OSTI ID:
- 6429830
- Journal Information:
- J. Bacteriol.; (United States), Vol. 169:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium
Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HYDROGEN PEROXIDE
SYNTHESIS
LIGNIN
METABOLISM
PEROXIDASES
ENZYME ACTIVITY
FUNGI
METABOLITES
CARBOHYDRATES
ENZYMES
HYDROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
PLANTS
POLYSACCHARIDES
SACCHARIDES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550200 - Biochemistry