Hypothesis: cyclic AMP turnover in S49 cells
The fractional turnover constant (ke) of cAMP in S49 lymphoma cells stimulated by epinephrine was essentially identical to the decay constant for cAMP in these cells. This was in sharp distinction to the situation in the human diploid lung fibroblast WI-38, in which ke was much lower in hormone stimulated cells. In this study we report a new method for the determination of cAMP turnover. The method was based on the assumption that for tritium label to be incorporated into cAMP on treatment of cells with (/sup 3/H)-adenine, the label must first pass through ATP. An equation relating the rate of change in cAMP specific radioactivity with cAMP and ATP specific radioactivities was thereby determined. The equation was expressed in a form that gave a linear graphical plot with the fractional turnover constant as the slope of the line.
- Research Organization:
- Graduate School of Biomedical Sciences, University of Texas Health Science Center at Houston
- OSTI ID:
- 6427530
- Journal Information:
- J. Cyclic Nucleotide Res.; (United States), Vol. 7:6
- Country of Publication:
- United States
- Language:
- English
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AMP
BIOCHEMICAL REACTION KINETICS
LYMPHOMAS
BIOCHEMISTRY
TRITIUM COMPOUNDS
ISOTOPE APPLICATIONS
ADENINES
ADRENALINE
ATP
TUMOR CELLS
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANTIMETABOLITES
AUTONOMIC NERVOUS SYSTEM AGENTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMISTRY
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HORMONES
KINETICS
LABELLED COMPOUNDS
NEOPLASMS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
REACTION KINETICS
STEROID HORMONES
SYMPATHOMIMETICS
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