Characterization of chicken c-ski oncogene products expressed by retrovirus vectors

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.6.3137· OSTI ID:6423221

Sutrave, P; Copeland, T D; Hughes, S H ^[1]; Showalter, S D ^[2]

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1. Advanced BioScience Lab. Inc., National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD (US)
2. Program Resources Inc., National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD (US)

The authors have constructed replication-competent avian retrovirus vectors that contain two of the three known types of chicken c-{ital ski} cDNAs and a third vector that contains a truncated c-{ital ski} cDNA. They developed antisera that recognize the c-{ital ski} proteins made by the three transforming c-{ital ski} viruses. All three proteins (apparent molecular masses, 50, 60, and 90 kilodaltons) are localized primarily in the nucleus. The proteins are differentially phosphorylated; immunofluorescence also suggests that there are differences in subnuclear localization of the c-{ital ski} proteins and that c-{ital ski} protein is associated with condensed chromatin in dividing cells.

• OSTI ID: 6423221
• Journal Information: Molecular and Cellular Biology; (USA), Vol. 10:6; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE RECOMBINATION PROTEINS
IMMUNOASSAY
ONCOGENES
MOLECULAR BIOLOGY
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL MODELS
CELL PROLIFERATION
CHROMATIN
DNA REPLICATION
DNA-CLONING
EXPERIMENTAL DATA
FLUORESCENCE
ONCOGENIC VIRUSES
PHENOTYPE
PHOSPHORYLATION
BIOASSAY
CHEMICAL REACTIONS
CLONING
DATA
DNA HYBRIDIZATION
GENES
HYBRIDIZATION
INFORMATION
LUMINESCENCE
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
PROTEINS
VIRUSES
550200* - Biochemistry
550400 - Genetics