Extractive fermentation for enhanced propionic acid production from lactose by Propionibacterium acidipropionici
Abstract
An extractive fermentation process using an amine extractant and a hollow-fiber membrane extractor to selectively remove propionic acid from the fermentation broth was developed to produce propionate from lactose. Compared to the conventional batch fermentation, the extractive fermentation had a much higher productivity ({approximately}1 g/(L{center_dot}h) or 5-fold increase), higher propionate yield (up to 0.66 g/g or more than 20% increase), higher final product concentration (75 g/L or higher), and higher product purity ({approximately}90%). Meanwhile, acetate and succinate productions in the extractive fermentation were significantly reduced. The improved fermentation performance can be attributed to the reduced product inhibition and a possible metabolic pathway shift to favor more propionic but less acetic and succinic acid production. The process was stable and gave consistent long-term performance over the 1.5-month period studied. The effects of propionate concentration, pH, and amine content in the solvent on the extractive fermentation were also studied and are discussed in this paper.
- Authors:
-
- Ohio State Univ., Columbus, OH (United States). Dept. of Chemical Engineering
- Publication Date:
- OSTI Identifier:
- 642292
- Resource Type:
- Journal Article
- Journal Name:
- Biotechnology Progress
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 3; Other Information: PBD: May-Jun 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; FERMENTATION; PROPIONIC ACID; LACTOSE; BACTERIA; BIOSYNTHESIS; YIELDS
Citation Formats
Jin, Z, and Yang, S T. Extractive fermentation for enhanced propionic acid production from lactose by Propionibacterium acidipropionici. United States: N. p., 1998.
Web. doi:10.1021/bp980026i.
Jin, Z, & Yang, S T. Extractive fermentation for enhanced propionic acid production from lactose by Propionibacterium acidipropionici. United States. https://doi.org/10.1021/bp980026i
Jin, Z, and Yang, S T. 1998.
"Extractive fermentation for enhanced propionic acid production from lactose by Propionibacterium acidipropionici". United States. https://doi.org/10.1021/bp980026i.
@article{osti_642292,
title = {Extractive fermentation for enhanced propionic acid production from lactose by Propionibacterium acidipropionici},
author = {Jin, Z and Yang, S T},
abstractNote = {An extractive fermentation process using an amine extractant and a hollow-fiber membrane extractor to selectively remove propionic acid from the fermentation broth was developed to produce propionate from lactose. Compared to the conventional batch fermentation, the extractive fermentation had a much higher productivity ({approximately}1 g/(L{center_dot}h) or 5-fold increase), higher propionate yield (up to 0.66 g/g or more than 20% increase), higher final product concentration (75 g/L or higher), and higher product purity ({approximately}90%). Meanwhile, acetate and succinate productions in the extractive fermentation were significantly reduced. The improved fermentation performance can be attributed to the reduced product inhibition and a possible metabolic pathway shift to favor more propionic but less acetic and succinic acid production. The process was stable and gave consistent long-term performance over the 1.5-month period studied. The effects of propionate concentration, pH, and amine content in the solvent on the extractive fermentation were also studied and are discussed in this paper.},
doi = {10.1021/bp980026i},
url = {https://www.osti.gov/biblio/642292},
journal = {Biotechnology Progress},
number = 3,
volume = 14,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1998},
month = {Fri May 01 00:00:00 EDT 1998}
}