Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:6422065
- Univ. of Tennessee, Knoxville (USA)
Purified flagella from two strains of {sup 32}P-labeled Pseudomonas aeruginosa were shown to be phosphorylated. This was confirmed by autoradiography of flagellin protein in polyacrylamide gels. Thin-layer electrophoresis and autoradiography of flagellin partial hydrolysates indicated that phosphotyrosine was the major phosphorylated amino acid. High-pressure liquid chromatographic analysis confirmed the presence of phosphotyrosine in flagellum filament protein. Preliminary data indicated that less than one tyrosine per subunit was phosphorylated. No evidence was found for phosphorylation of serine or threonine. A function related to tyrosine phosphorylation has not been determined.
- OSTI ID:
- 6422065
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 172:9; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
TYROSINE
PHOSPHORYLATION
AUTORADIOGRAPHY
ELECTROPHORESIS
LIQUID COLUMN CHROMATOGRAPHY
MOLECULAR WEIGHT
ORGANOIDS
PHOSPHORUS 32
PSEUDOMONAS
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SEPARATION PROCESSES
550501* - Metabolism- Tracer Techniques
550701 - Microbiology- Tracer Techniques
TYROSINE
PHOSPHORYLATION
AUTORADIOGRAPHY
ELECTROPHORESIS
LIQUID COLUMN CHROMATOGRAPHY
MOLECULAR WEIGHT
ORGANOIDS
PHOSPHORUS 32
PSEUDOMONAS
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SEPARATION PROCESSES
550501* - Metabolism- Tracer Techniques
550701 - Microbiology- Tracer Techniques