Solvent-induced autolysis of cells
A process is described for the recovery of alcohol oxidase from whole cells of Pichia pastoris grown on methanol which comprises (a) forming an aqueous mixture of the whole cells, wherein the cells are present in an amount of from 85 to 150 grams per liter of aqueous mixture and from 0.8 to 6 volume percent of a treating agent selected from the group consisting of chloroform, 1,1,1-trichloroethane and methylene chloride or mixtures thereof, and wherein, the aqueous mixture has a pH of about 6.5 to about 8.5; (b) incubating the aqueous mixture at a temperature of about 20/sup 0/ to 35/sup 0/C for a time of about 16 to 90 hours whereby at least a portion of the alcohol oxidase is released from the cells thereby forming a alcohol oxidase-containing aqueous liquor; and (c) separating alcohol oxidase-containing liquor liquid from solid cell material.
- Assignee:
- Phillips Petroleum Co., Bartlesville, OK
- Patent Number(s):
- US 4795709
- OSTI ID:
- 6421870
- Resource Relation:
- Patent File Date: Filed date 10 Jun 1985
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALCOHOLS
RECOVERY
METHANOL
BIOCONVERSION
OXIDOREDUCTASES
PLANT CELLS
AQUEOUS SOLUTIONS
AUTOLYSIS
CHLOROFORM
ETHANE
GROWTH
INCUBATION
MEDIUM TEMPERATURE
METHYLENE CHLORIDE
PRODUCTION
SEPARATION PROCESSES
SOLVENTS
ALKANES
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
DISPERSIONS
ENZYMES
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
HYDROXY COMPOUNDS
MIXTURES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
SOLUTIONS
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