Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro
- Keio Univ. School of Medicine, Tokyo (Japan)
Bone marrow is one of the most radiosensitive organs. Irradiation causes a marked decrease in the total number of hematopoietic cells in the bone marrow. The reticular meshwork structure of marrow stromal cells, however, is relatively resistant to irradiation. Unimpaired stromal cell structure has been thought to be a prerequisite for the repopulation of hematopoietic cells during recovery from the effects of irradiation. The reticular framework is maintained by cell adhesion apparatuses such as gap junctions. The in vitro radiobiologic survival values of a cloned stromal cell line, H-1/A, were studied (n = 1.8, D0 = 138 cGy). Radiation doses of up to 4000 cGy had no detectable effects on the production of colony-stimulating factor 1. H-1/A cells communicate with each other via gap junctions as determined by the sensitive dye-transfer method. Gap-junctional communication between H-1/A cells was resistant to different levels of irradiation (500 to 10,000 cGy), but it was lost during adipocyte differentiation of H-1/A cells. Marrow stromal cells, which are important in the recovery of hematopoiesis, seemed capable of coordination with each other through gap junctions even when exposed to radiation.
- OSTI ID:
- 6420159
- Journal Information:
- Experimental Hematology (Lawrence, Kansas); (USA), Vol. 18:9; ISSN 0301-472X
- Country of Publication:
- United States
- Language:
- English
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BONE MARROW CELLS
RADIOSENSITIVITY
CLONE CELLS
COLONY FORMATION
RADIATION DOSES
ANIMAL CELLS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DOSES
SOMATIC CELLS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture