Pharmacological control of mucociliary activity in the gill of Mytilus edulis
A series of studies has been carried out, which include the development of a radiolabelling technique for mucous secretion studies, the pharmacological mediation of mucous secretion and ciliary activity, and the identification and localization of cAMP activity in different cell types in the gill filament. A radiolabelling technique was developed using ({sup 14}C)glucosamine as a precursor for the biosynthesis of mucous glycoproteins by the gill tissues. Selective incorporation of the radiolabel into mucous glycoproteins was evident from autoradiographic studies. The mucous secretion was significantly stimulated by serotonin, forskolin, and acetylcholine, and these effects were enhanced or blocked, respectively, by their agonists or antagonists. Interestingly, the stimulatory effect of serotonin on mucous secretion was inhibited by 10{sup {minus}4}M atropine, a cholinergic antagonist, suggesting that the mucous secretion is directly mediated through acetylcholine-induced action rather than the cAMP second messenger system. To further substantiate this hypothesis, a histochemical localization of cAMP in the gill tissue sections was carried out using a horseradish peroxidase conjugated antibody method.
- Research Organization:
- West Virginia Univ., Morgantown, WV (USA)
- OSTI ID:
- 6415058
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMP
BIOLOGICAL LOCALIZATION
GLYCOPROTEINS
SECRETION
MUSSELS
BIOCHEMISTRY
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
GILLS
GLUCOSAMINE
MUCOUS MEMBRANES
PEROXIDASES
PRECURSOR
SEROTONIN
AMINES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
CARBOHYDRATES
CHEMISTRY
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
HEXOSAMINES
HEXOSES
HYDROXY COMPOUNDS
INDOLES
INVERTEBRATES
KINETICS
LABELLED COMPOUNDS
MEMBRANES
MOLLUSCS
MONOSACCHARIDES
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RESPIRATORY SYSTEM
SACCHARIDES
SYMPATHOMIMETICS
SYNTHESIS
TRYPTAMINES
550201* - Biochemistry- Tracer Techniques