Nematode cholinergic pharmacology

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6415004

Segerberg, M A

Nematode acetylcholine (ACh) receptors were characterized using both biochemical and electrophysiological techniques, including: (1) receptor binding studies in crude homogenates of the free-living nematode Caenorhabditis elegans and the parasitic nematode Ascaris lumbricoides with the high-affinity probe ({sup 3}H)N-methylscopolamine (({sup 3}H)NMS) which binds to muscarinic receptors in many vertebrate and invertebrate tissues (2) measurement of depolarization and contraction induced by a variety of cholinergic agents, including N-methylscopolamine (NMS), in an innervated dorsal muscle strip preparation of Ascaris; (3) examination of the antagonistic actions of d-tubocurarine (dTC) and NMS at dorsal neuromuscular junction; (4) measurement of input resistance changes in Ascaris commissural motorneurons induced by ACh, dTC, NMS, pilocarpine and other cholinergic drugs.

• Research Organization: Wisconsin Univ., Madison, WI (USA)
• OSTI ID: 6415004
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
RECEPTORS
NEMATODES
ELECTROPHYSIOLOGY
CHEMICAL COMPOSITION
BIOCHEMICAL REACTION KINETICS
MUSCLES
NERVE CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ASCHELMINTHES
AUTONOMIC NERVOUS SYSTEM AGENTS
DRUGS
ESTERS
HELMINTHS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PHYSIOLOGY
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques