Cell retention culture with an internal filter module: Continuous ethanol fermentation

Journal Article · Mon Mar 15 00:00:00 EST 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260410612· OSTI ID:6414919

Chang, Ho Nam; Lee, Woo Gi; Kim, Beom Soo ^[1]

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1. Korea Advanced Inst. of Science and Technology, Taejon (Korea, Republic of)

A new internal filter feedback system with a stainless steel filter was introduced and its application for continuous ethanol fermentation was investigated. The filter performance was highly influenced by agitation speed and yeast concentration. Retention coefficient with a filter of 2 [mu]m pore size was found more than 97.5%, and the filter was suitable for yeast separation. Maximum yeast concentration was 157 g/L and the best operable cell concentration was between 90 and 150 g/L, which was similar to that obtained in the external membrane cell recycle culture. The cell concentration in the fermentor was maintained by manipulation of dilution rate and bleed ratio with the growth rate. The internal filter feedback system was successfully operated for more than 10 days. The study shows that the internal filter feedback system with a stainless steel filter can be used as successfully as an external cell recycle system for high-density cell culture and ethanol fermentation. Furthermore, it can be scaled up more easily than the external cell recycle system.

• OSTI ID: 6414919
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 41:6; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ETHANOL
YIELDS
GLUCOSE
FERMENTATION
YEASTS
GROWTH
BIOREACTORS
CELL CULTURES
CONTINUOUS CULTURE
FILTERS
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
EUMYCOTA
FUNGI
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
090900* - Biomass Fuels- Processing- (1990-)