Characterization of solubilized human and rat brain US -endorphin-receptor complex
Opioid receptors have been solubilized from human striatal and rat whole-brain membranes by use of 3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS). Tritiated human US -endorphin (TH-US /sub h/-EP) binding revealed high-affinity competition by morphine, naloxone, and various US -EP analogues. Lack of high-affinity competition by (+/-)-3,4-dichloro-N-methyl-N-(2-(1-pyrrolidinyl)cyclohexyl)benzeneacetamide methanesulfonate (U50-488, Upjohn) indicated that k sites were not labeled by TH-US -/sub h/-EP under these conditions. Affinities were similar in both soluble and membrane preparations except for (Met)enkephalin, which appears to be rapidly degraded by the solubilized extract. Size differences between human and rat solubilized TH-US /sub h/-EP-receptor complexes were revealed by exclusion chromatography.
- Research Organization:
- Univ. of California, San Francisco, CA (United States)
- OSTI ID:
- 6413820
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Functional reconstitution of receptors for bradykinin and des argZ-bradykinin from pulmonary artery membranes
Biochemical and immunological studies of the Muscarinic acetylcholine receptor
Related Subjects
ENDORPHINS
RADIORECEPTOR ASSAY
BRAIN
CELL MEMBRANES
MAN
MOLECULAR STRUCTURE
RATS
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DRUGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANS
PRIMATES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics