Sensitive radioimmunoassay of. cap alpha. human atrial natriuretic polypeptide using monoclonal antibody recognizing human form ring structure

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90176-2· OSTI ID:6412379

Naomi, S; Umeda, T; Iwaoka, T; Miura, F; Ohno, M; Sasaki, M; Oishi, S; Sato, T; Takatsu, K

A monoclonal antibody (C351) against ..cap alpha.. human atrial natriuretic polypeptide (..cap alpha..hANP) recognizing human form ring structure was established and applied to a radioimmunoassay of plasma ..cap alpha..hANP. The minimum detectable amount in terms of 10 % radioligand displacement relative to zero dose were 0.28 fmol/tube, corresponding to 0.7 fmol/ml in plasma after extraction using Sep-Pak C18 cartridges. When the mean plasma levels at recumbent position in fasted morning were compared in 10 young (<30 years) and 10 elderly (greater than or equal to50 years) healthy subjects taking normal sodium diet, it was slightly higher in the latter. After i.v. infusion of hypertonic saline at a rate of 0.24 ml/kg/min for 20 min in 6 normal subjects (26 to 35 years), it was increased from 4.1 +/- 0.4 to 5.9 +/- 0.7 fmol/ml (p<0.01). In 6 patients with essential hypertension (34 to 57 years), it was elevated with high salt intake. From these results, the radioimmunoassay of plasma IR-..cap alpha..hANP using MAb C351 seems to be quite suitable to detect rather small changes at low plasma concentrations and to investigate a physiological importance of ..cap alpha..hANP in man.

• Research Organization: Kumamoto Univ. Medical School (Japan)
• OSTI ID: 6412379
• Journal Information: Life Sci.; (United States), Vol. 43:9
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
POLYPEPTIDES
MOLECULAR STRUCTURE
RADIOIMMUNOASSAY
AGE DEPENDENCE
MAN
MONOCLONAL ANTIBODIES
ANIMALS
ANTIBODIES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
ORGANIC COMPOUNDS
PEPTIDES
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550201* - Biochemistry- Tracer Techniques