Inhibition of Na -P/sub i/ cotransporter in small gut brush border by phosphonocarboxylic acids

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:6411864

Loghman-Adham, M; Szczepsanska-Konkel, M; Yusufi, A N.K.; Van Scoy, M; Dousa, T P

The authors examined the effect of phosphonoformic acid (PFA) and phosphonoacetic acid (PAA) upon Na -P/sub i/ cotransport in brush-border membrane (BBM) from small gut of rat. Both PFA and PAA inhibited the Na gradient-dependent uptake of TSP/sub i/ by BBM vesicles (BBMV) prepared from intestinal mucosa but had no effect on Na -dependent uptakes of D-(TH)glucose, L-(TH)proline, or ( UC)succinate. The uptake in the absence of Na gradient, or uptake at equilibrium period (180 min), was not affected by PFA or by PAA. A chemical analogue of PFA and PAA, phosphonopropionic acid, had only a minor inhibitory effect and phenylphosphonic acid was inactive. Neither PFA nor PAA influenced the activity of rat intestinal BBM alkaline phosphatase. The BBMV from rat jejunum had a much higher capacity for Na gradient-dependent uptake of TSP/sub i/ than BBMV from duodenum or ileum. The inhibition of BBMV TSP/sub i/ transport across rat jejunum by PFA is competitive. They suggest that PFA and PAA are specific inhibitors of Na gradient-dependent uptake of P/sub i/ by BBMV from small intestinal mucosa and that they could serve as useful experimental tools for the studies of intestinal Na -P/sub i/ cotransport.

• Research Organization: Mayo Clinic and Foundation, Rochester, MN
• OSTI ID: 6411864
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:2
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
BIOCHEMISTRY
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
PHOSPHORUS 32
ACETIC ACID
CARBON 14 COMPOUNDS
CATIONS
FORMIC ACID
GLUCOSE
MUCOUS MEMBRANES
PHOSPHONIC ACIDS
PROLINE
SMALL INTESTINE
SODIUM COMPOUNDS
SUCCINIC ACID
TRITIUM COMPOUNDS
ALDEHYDES
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CHARGED PARTICLES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DICARBOXYLIC ACIDS
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
INTESTINES
IONS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PYRROLES
PYRROLIDINES
RADIOISOTOPES
SACCHARIDES
551001* - Physiological Systems- Tracer Techniques