Genetic control of the humoral immune response to avian egg white lysozymes in the chicken

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6411643

Flanagan, M P

Chickens from two closely related sublines, GHs-B6 and GHs-B13, differing serologically at the major histocompatibility complex, were significantly different in their humoral response to three avian egg white lysozymes. Specific antisera levels were measured by radioimmunoassay using /sup 125/I-labeled lysozymes. Antibodies elicited in response to these lysozymes are assumed to be directed against sites on these lysozymes where their amino acid sequence differs from that of the recipient G. domesticus egg white lysozyme (HEL). GHs-B6 birds produced a high level of antibody in response to immunization of turkey (TEL), pheasant (PhL) and guinea hen (GHL) lysozymes. GHs-B13 birds produced no detectable antibody to TEL, were intermediate in their response to PhL and equaled the antibody production of GHs-B6 birds in response to GHL. Antisera to each lysozyme were examined for crossreactivity with all other lysozymes by use of a competitive binding assay.

• Research Organization: Iowa State Univ. of Science and Technology, Ames (USA)
• OSTI ID: 6411643
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHICKENS
IMMUNE REACTIONS
LYSOZYME
AMINO ACID SEQUENCE
ANTIGEN-ANTIBODY REACTIONS
GENETIC VARIABILITY
IODINE 125
RADIOIMMUNOASSAY
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
BIRDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FOWL
GLYCOSYL HYDROLASES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MOLECULAR STRUCTURE
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques