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Title: Clean fuel from bioconversion of solar energy

Technical Report ·
OSTI ID:6408893

Investigating the use of unicellular algae to produce glycolic acid for subsequent conversion to methane by anaerobic digestion, SRC (1) evaluated a defined medium that supports rapid autotrophic algae growth, (2) estimated the glycolic acid production rates of four genera of algae, choosing Chlorella pyrenoidosa and Chlamydomonas reinhardtii for further study, (3) determined the effects of temperature, pH, light source and intensity, and atmospheric CO/sub 2/ concentration on glycolic acid excretion of C. pyrenoidosa, (4) demonstrated the influence of varing CO/sub 2/ concentrations on the growth and glycolic acid production of C. pyrenoidosa and C. reinhardtii, (5) developed a procedure for separating and quantitating gylcolic acid in a culture medium, and (6) introduced a method of screening and isolating mutants of C. reinhardtii that produce more glycolic acid. Test results recommend further study of isolated mutants of C. reinhardtii in order to optimize the physiological conditions that would result in high levels of glycolic acid, and also exploration of the abiotic formation of formaldehyde from glycolic acid as another route to a usable fuel.

Research Organization:
Syracuse Research Corp., NY (USA)
OSTI ID:
6408893
Report Number(s):
PB-83-136226
Country of Publication:
United States
Language:
English