Role of calcium-dependent proteinase in molt-induced claw muscle atrophy

Conference · Sun Jan 01 00:00:00 EST 1984

OSTI ID:6408439

Mykles, D L; Skinner, D M

The claw closer muscle of the Bermuda land crab Gecarcinus lateralis undergoes a sequential atrophy and restoration during each intermolt cycle. Muscle protein decreases 40% during proecdysis and is restored following ecdysis. Amino acid incorporation into protein of postecdysial muscle is five times greater than that in anecdysial muscle. Since the rates of protein synthesis in anecdysial and proecdysial muscle are the same it appears that proecdysial muscle atrophy is caused primarily by an increase in protein degradation. A calcium-dependent proteinase (CDP) active at neutral pH has been implicated in the nonlysosomal hydrolysis of myofibrillar proteins. We have examined the role of a CDP in atrophy of the claw closer muscle. The many similarities between crustacean and vertebrate CDPs have established this crustacean system as a simple and convenient model for the role of Ca/sup 2 +/-dependent proteolysis in myofibrillar protein turnover and its manifestation in the structure of the sarcomere. 16 references, 8 figures. (ACR)

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6408439
• Report Number(s): CONF-8405243-1; ON: DE85001275
• Resource Relation: Conference: 5. international symposium on intracellular protein catabolism, Airlie, VA, USA, 29 May 1984
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATROPHY
PHYSIOLOGY
MUSCLES
CALCIUM
CRABS
ENZYME ACTIVITY
PEPTIDE HYDROLASES
ULTRASTRUCTURAL CHANGES
ALKALINE EARTH METALS
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
CRUSTACEANS
DECAPODS
ELEMENTS
ENZYMES
HYDROLASES
INVERTEBRATES
METALS
MORPHOLOGICAL CHANGES
PATHOLOGICAL CHANGES
551000* - Physiological Systems