Genetic transformation in the methanogen Methanococcus voltae PS

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:6405345

Bertani, G; Baresi, L

Mutations causing requirements for histidine, purine, and vitamin B/sub 12/ were obtained in strain PS of Methanococcus voltae (archaebacteria) upon irradiation with UV or gamma rays. The first two mutations were shown to revert at low frequencies and were sued to demonstrate the occurrence of transformation with homologous, wild-type DNA. The transformation rates obtained for these presumably chromosomal markers were in the range of 2 to 100 transformants per ..mu..g of DNA. Mutants resistant to 2-bromoethanesulfonate and to 5-methyl-DL-tryptophan were also isolated.

• Research Organization: California Institute of Technology, Pasadena
• OSTI ID: 6405345
• Journal Information: J. Bacteriol.; (United States), Vol. 169:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
METHANOGENIC BACTERIA
GENETIC RADIATION EFFECTS
SULFONATES
GENETIC EFFECTS
TRYPTOPHAN
CELL TRANSFORMATIONS
DNA
GAMMA RADIATION
MUTANTS
ULTRAVIOLET RADIATION
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRROLES
RADIATION EFFECTS
RADIATIONS
560130* - Radiation Effects on Microorganisms
560300 - Chemicals Metabolism & Toxicology