Identification of a non-specific inhibitor of (methyl/sup 3/H)thymidine incorporation into DNA of normal and pre-neoplastic cells

Conference · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6400688

Overbye, L J

Growth in mammalian cells is controlled by a variety of factors, both stimulatory and inhibitory. Bullough et al. (2) first proposed the idea that endogenous mitotic inhibitors may play a role in controlling cellular proliferation. Inhibitors may be tissue-specific (9) or non-specific (for review see Lozzio et al. (5)). Although many inhibitors are proteins of high molecular weights, several low molecular weight inhibitors of (/sup 3/H) thymidine incorporation (and therefore DNA synthesis) have been identified (1,3,6). Unlabelled thymidine has also been noted to be inhibitory (4). This paper describes a non-specific, unstable inhibitor that is found in media conditioned by neoplastic epithelial cells and some normal fibroblasts and inhibits (/sup 3/H) thymidine incorporation into normal and pre-neoplastic cells. The inhibitory activity has not been isolated and could consists of more than one compound. 9 refs., 1 tab.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6400688
• Report Number(s): CONF-8905121-1; ON: DE89010372
• Resource Relation: Conference: National conference of undergraduate research, San Antonio, TX, USA, 1 May 1989; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL GROWTH
INHIBITION
DNA
LABELLING
ENZYME INHIBITORS
UPTAKE
ANIMAL CELLS
NEOPLASMS
THYMIDINE
AZINES
DISEASES
GROWTH
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
550602* - Medicine- External Radiation in Diagnostics- (1980-)