Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors

Journal Article · Thu Dec 01 00:00:00 EST 1988 · J. Virol.; (United States)

OSTI ID:6399081

Johnson, D C; Ligas, M W

Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cell pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.

• Research Organization: McMaster Univ., Hamilton, ON (Canada)
• OSTI ID: 6399081
• Journal Information: J. Virol.; (United States), Vol. 62:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMISTRY
VIRUSES
RECEPTORS
ANIMAL CELLS
EXTREME ULTRAVIOLET RADIATION
HERPES SIMPLEX
INHIBITION
MAN
MONOCLONAL ANTIBODIES
MUTATIONS
ANIMALS
ANTIBODIES
CARBOHYDRATES
CHEMISTRY
DISEASES
ELECTROMAGNETIC RADIATION
GLYCOPROTEINS
INFECTIOUS DISEASES
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
PROTEINS
RADIATIONS
SACCHARIDES
SKIN DISEASES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
550701* - Microbiology- Tracer Techniques