ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae
- Harvard Medical School, Boston, MA (United States)
The genes encoding three lipoproteins of Vibrio cholerae were identified by a combination of DNA sequence analysis and ({sup 3}H)palmitate labeling of hybrid proteins encoded by TnphoA gene fusions. The expression of these three lipoproteins, TagA, AcfD, and TcpC, was controlled by ToxR, the cholera toxin transcriptional activator. The involvement of other bacterial lipoproteins in conferring resistance ot the bactericidal effects of complement prompted us to examine this possibility in V. cholerae. Remarkably, mutations in toxR and tcp genes (including tcpC), involved in the biogenesis of the toxin coregulated pili, rendered V. cholerae about 10{sup 4} - 10{sup 6} times more sensitive to the vibriocidal activity of antibody and complement. Since V. cholerae is a noninvasive organism and toxR and tcp mutants are highly defective in intestinal colonization in animals and humans, these results raise the possibility that resistance to a gut-associated, complement-like bactericidal activity may be a major virulence determinant of V. cholerae and other enterobacterial species.
- OSTI ID:
- 6397825
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:5; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHOLERA
PATHOGENESIS
GENES
DNA-CLONING
LIPOPROTEINS
BIOSYNTHESIS
TOXINS
BIOLOGICAL EFFECTS
BACTERIA
CELL MEMBRANES
COMPLEMENT
ELECTROPHORESIS
ANTIGENS
BACTERIAL DISEASES
CELL CONSTITUENTS
CLONING
DISEASES
DNA HYBRIDIZATION
HYBRIDIZATION
INFECTIOUS DISEASES
LIPIDS
MATERIALS
MEMBRANES
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
SYNTHESIS
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques