Liquid chromatographic determination of benzo(a)pyrene in natural, synthetic, and refined crudes
Benzo(a)pyrene (BaP) is isolated and quantitated by using a sequential high-pressure liquid chromatographic (HPLC) procedure. The sample is first injected onto a semipreparative HPLC column containing a bonded polar aminocyano (PAC) packing material, from which a BaP-enriched fraction is obtained. This isolate is then reinjected onto a Zorbax ODS reversed-phase analytical-scale column, and fluorescence detection is used to quantitate BaP. This procedure is applicable to samples with BaP concentrations ranging from 0.02 to 500 ..mu..g/g. The precision is nominally +- 6% (relative standard deviation), and the accuracy compares favorably with that displayed by more tedious methods. Recoveries, as determined by counting a radioactive BaP tracer, usually exceed 95%. Two samples may be processed per person-day.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6396201
- Journal Information:
- Anal. Chem.; (United States), Vol. 53:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
01 COAL, LIGNITE, AND PEAT
04 OIL SHALES AND TAR SANDS
02 PETROLEUM
BENZOPYRENE
FLUORESCENCE SPECTROSCOPY
LIQUID COLUMN CHROMATOGRAPHY
COAL LIQUIDS
FRACTIONATION
PETROLEUM
SHALE OIL
TAR
ACCURACY
COLUMN PACKING
QUANTITATIVE CHEMICAL ANALYSIS
SENSITIVITY
SYNTHETIC FUELS
AROMATICS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
EMISSION SPECTROSCOPY
ENERGY SOURCES
FLUIDS
FOSSIL FUELS
FUELS
HYDROCARBONS
LIQUIDS
MINERAL OILS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PACKINGS
SEPARATION PROCESSES
SPECTROSCOPY
400105* - Separation Procedures
400104 - Spectral Procedures- (-1987)
010600 - Coal
Lignite
& Peat- Properties & Composition
040500 - Oil Shales & Tar Sands- Properties & Composition
023000 - Petroleum- Properties & Composition