Carbon monoxide effects on calcium levels in vascular smooth muscle
Previously the authors showed that carbon monoxide (CO) relaxes vascular smooth muscle in the working heart and thoracic aorta preparation perfused with hemoglobin-free, Krebs-Henseleit (KH) solution. The CO-induced relaxation was not caused by hypoxia, nor was it mediated by adrenergic influences, adenosine, or prostaglandins. In these studies the effect of CO on calcium (Ca/sup + +/) concentrations in vascular smooth muscle was determined using /sup 45/Ca as a tracer. Isolated rat thoracic aorta segments were incubated with /sup 45/Ca and gassed with O/sub 2/, N/sub 2/, or CO for 60 min. Verapamil was used to verify the effectiveness of the test system. Ca/sup + +/ concentrations were 488 /+ -/ 35 and 515 /+ -/ 26 mM/g tissue (X /+ -/ SE) in aortic rings gassed with O/sub 2/ and N/sub 2/, respectively. CO reduced Ca/sup + +/ concentrations significantly (P<0.01) by 29% to 369 /+ -/ 18 mM/g tissue. Verapamil treatment reduced Ca/sup + +/ concentrations by 40% to 314 /+ -/ 23 mM/g tissue. These results suggest that CO relaxes vascular smooth muscle and dilates blood vessels by decreasing Ca/sup + +/ concentrations in vascular smooth muscle.
- Research Organization:
- Texas Tech Univ. Health Sciences Center, Lubbock (USA)
- OSTI ID:
- 6395742
- Journal Information:
- Life Sci.; (United States), Vol. 43:22
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CALCIUM
BIOSYNTHESIS
CARBON MONOXIDE
BIOLOGICAL EFFECTS
AORTA
CALCIUM 45
MUSCLES
RATS
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CALCIUM ISOTOPES
CARBON COMPOUNDS
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALS
NUCLEI
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques