Single-hit potentially lethal damage: evidence of its repair in mammalian cells
Following mid to large doses of X rays, or of fission spectrum neutrons, the repair of potentially lethal damage in V79 Chinese hamster cells can be inhibited by anisotonic phosphate-buffered saline or by medium containing 90% D/sub 2/O. The foregoing post-treatments do not affect the viability of unirradiated cells. Using single synchronized cells irradiated in late S-phase, the most resistant phase of the cell cycle, repair of potentially lethal damage in late S-phase, the most resistant phase of the cell cycle, repair of potentially lethal damage in the single-hit, initially exponential, or small-dose part of the survival curve was examined. The use of synchronized cells avoids misinterpretations due to population heterogeneity. The slope of the small-dose, exponential region of the neutron survival curve is much steeper than that of the x-ray survival curve. Even so, it is demonstrated with post-treatments consisting of hypertonic phosphate-buffered saline, medium containing D/sub 2/O,adiation is connected with their proliferation but not with the migration out from lymphoid organs.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31-109-ENG-38
- OSTI ID:
- 6394714
- Journal Information:
- Radiat. Res.; (United States), Vol. 87:3; Conference: 28. annual scientific meeting of the Radiation Research Society, New Orleans, LA, USA, 4 Jun 1980
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
RADIATION INJURIES
BIOLOGICAL REPAIR
CAFFEINE
COMPARATIVE EVALUATIONS
HAMSTERS
NEUTRON SPECTRA
RADIATION DOSES
SURVIVAL CURVES
X RADIATION
ANALEPTICS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CENTRAL NERVOUS SYSTEM AGENTS
DOSES
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
INJURIES
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SPECTRA
VERTEBRATES
XANTHINES
560121* - Radiation Effects on Cells- External Source- (-1987)