Spontaneous mutants of the adenovirus-simian virus 40 hybrid, Ad2/sup +/ND3, that grow efficiently in monkey cells

Journal Article · Fri May 01 00:00:00 EDT 1981 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(81)90670-X· OSTI ID:6390107

Anderson, C W

An attempt was made to isolate spontaneous mutants of adenovirus type 2 and of the adenovirus-SV40 hybrids, Ad2/sup +/ND3 and Ad2/sup +/ND5, that would grow efficiently on monkey cells. Virus stocks were serially passaged through the semipermissive established monkey line CV-1. After five serial passages in the absence of intentional mutagenesis, only stocks of Ad2/sup +/ND3 yielded significant numbers of variants that plaqued with similar efficiency on human and on monkey cell monolayers. Four independent Ad2/sup +/ND3 variants, designated hr600, hr601, hr602, and hr603, have been isolated and partially characterized. No difference was found between the genomes of these variants and the genome of parental Ad2/sup +/ND3 by restriction enzyme analysis or by the analysis of heteroduplexes between Ad2/sup +/ND3 (or variant) DNA and DNA of the hybrid Ad2/sup +/ND1.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6390107
• Journal Information: Virology; (United States), Vol. 111:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
MUTANTS
ENZYME ACTIVITY
GENETIC VARIABILITY
POLYPEPTIDES
SIMIAN VIRUS
ANIMAL CELLS
BIOSYNTHESIS
DNA
HYBRIDIZATION
MONKEYS
PHENOTYPE
ANIMALS
BIOLOGICAL VARIABILITY
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES
VIRUSES
550700* - Microbiology
550400 - Genetics