Replication and matagenesis of UV-damaged DNA templates in human and monkey cell extracts
- Univ. of Cincinnati, Ohio (United States)
- National Institute of Child Health and Human Developement, Bethesda, MD (United States)
The mechanism by which ultraviolet radiation induced DNA damage causes mutations in humans is not well understood. Ultraviolet light has been shown to be mutagenic in a variety of repair-proficient and repair-deficient mammalian cells in culture. The frequency of mutations appears to correlate with the amount of unrepaired DNA damage remaining in the DNA at the time of replication. This suggests that replication of damaged DNA leads to mutation fixation. This research examines the mechanism of replication of UV-damaged templates and mutation fixation in an in-vitro, mammalian cell-free system. The establishment of this system offers new experimental approaches for gaining a detailed understanding of the process of mutation fixation at the molecular and biochemical levels.
- OSTI ID:
- 6385338
- Journal Information:
- Molecular and Cellular Biology; (United States), Vol. 13:1; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
Similar Records
Analysis of mutations induced by replication of UV-damaged plasmid DNA in HeLa cell extracts
In-vitro replication of UV-irradiated DNA by human cell extracts: Evidence that xeroderma pigmentosum variant (XP-V) cells bypass lesions in an abnormal, error-prone manner
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
MUTATIONS
ULTRAVIOLET RADIATION
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
DNA REPLICATION
GENES
MONKEYS
MUTAGENESIS
ANIMALS
ELECTROMAGNETIC RADIATION
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550400 - Genetics