Arachidonic acid stimulates /sup 45/calcium efflux and HPL release in isolated trophoblast cells
Previous investigations from this laboratory have indicated that arachidonic acid stimulates a rapid, dose-dependent and reversible increase in hPL release which is not dependent on cyclooxygenase or lipoxygenase metabolism. To investigate further the mechanism by which arachidonic acid stimulates the release of hPL, the effect of arachidonic acid on the release of /sup 45/Ca from perifused cells prelabelled with /sup 45/Ca was examined in an enriched cell culture population of term human syncytiotrophoblast. Arachidonic acid (10-100 ..mu..M) stimulated a dose-dependent, rapid, and reversible increase in the release of both /sup 45/Ca and hPL from the perifused placental cells. On the other hand, palmitic acid had little effect on either hPL release or /sup 45/Ca release even at concentrations as high as 100 ..mu..M. Ionophore A23187 (1-10..mu..M) also stimulated a dose-dependent and reversible increase in hPL release. Since arachidonic acid increases the mobilization of cellular calcium, as reflected by the increased /sup 45/calcium efflux, and since an increase in the intracellular calcium concentration appears to stimulate an increase in hPL release, these results suggest that the stimulation of hPL release by arachidonic acid may be due, at least in part, to the effects of the fatty acid on cellular calcium mobilization. 26 references, 5 figures.
- Research Organization:
- Duke Univ. Medical center, Durham, NC
- OSTI ID:
- 6381501
- Journal Information:
- Life Sci.; (United States), Vol. 38:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
CALCIUM
MEMBRANE TRANSPORT
HPL
SECRETION
ANIMAL CELLS
CALCIUM 45
CALCIUM CHLORIDES
CARBOXYLIC ACIDS
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
LABELLED COMPOUNDS
MAN
PLACENTA
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CALCIUM ISOTOPES
CHLORIDES
CHLORINE COMPOUNDS
DATA
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FETAL MEMBRANES
HALIDES
HALOGEN COMPOUNDS
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LACTOGENS
MAMMALS
MEMBRANES
METALS
MONOCARBOXYLIC ACIDS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIOISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques