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Title: Some considerations in the experimental approach to distinguishing between membrane transport and intracellular disposition of antineoplastic agents, with specific reference to fluorodeoxyuridine, actinomycin D, and methotrexate

Abstract

Analysis of the interaction between an anticancer agent and the intact cell can provide important clues to cytotoxic determinants and mechanisms of drug selectivity and resistance. This can be very useful in optimally evaluating single and multidrug regimens in test systems, designing new agents, and evaluating drug metabolism and binding to cellular target sites. This cellular pharmacokinetic approach requires very meticulous attention to experimental design, especially the time dimension chosen for study, and an appropriate framework for data analysis. This paper reviews cellular pharmacokinetic principles and experimental approaches that can be applied in uptake studies with cytotoxic agents, focusing, as examples, on studies from this laboratory with fluorodeoxyuridine, actinomycin D, and methotrexate.

Authors:
; ;
Publication Date:
Research Org.:
Department of Medicine, Medical College of Virginia, Richmond
OSTI Identifier:
6377257
Resource Type:
Journal Article
Journal Name:
Conn. Med.; (United States)
Additional Journal Information:
Journal Volume: 65 Suppl 3
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ANTINEOPLASTIC DRUGS; BIOLOGICAL PATHWAYS; PHARMACOLOGY; BIOCHEMICAL REACTION KINETICS; METABOLISM; TOXICITY; TUMOR CELLS; ANIMAL CELLS; DRUGS; KINETICS; REACTION KINETICS; 550600* - Medicine; 550200 - Biochemistry; 550900 - Pathology

Citation Formats

Goldman, D, Bowen, D, and Gewirtz, D A. Some considerations in the experimental approach to distinguishing between membrane transport and intracellular disposition of antineoplastic agents, with specific reference to fluorodeoxyuridine, actinomycin D, and methotrexate. United States: N. p., 1981. Web.
Goldman, D, Bowen, D, & Gewirtz, D A. Some considerations in the experimental approach to distinguishing between membrane transport and intracellular disposition of antineoplastic agents, with specific reference to fluorodeoxyuridine, actinomycin D, and methotrexate. United States.
Goldman, D, Bowen, D, and Gewirtz, D A. 1981. "Some considerations in the experimental approach to distinguishing between membrane transport and intracellular disposition of antineoplastic agents, with specific reference to fluorodeoxyuridine, actinomycin D, and methotrexate". United States.
@article{osti_6377257,
title = {Some considerations in the experimental approach to distinguishing between membrane transport and intracellular disposition of antineoplastic agents, with specific reference to fluorodeoxyuridine, actinomycin D, and methotrexate},
author = {Goldman, D and Bowen, D and Gewirtz, D A},
abstractNote = {Analysis of the interaction between an anticancer agent and the intact cell can provide important clues to cytotoxic determinants and mechanisms of drug selectivity and resistance. This can be very useful in optimally evaluating single and multidrug regimens in test systems, designing new agents, and evaluating drug metabolism and binding to cellular target sites. This cellular pharmacokinetic approach requires very meticulous attention to experimental design, especially the time dimension chosen for study, and an appropriate framework for data analysis. This paper reviews cellular pharmacokinetic principles and experimental approaches that can be applied in uptake studies with cytotoxic agents, focusing, as examples, on studies from this laboratory with fluorodeoxyuridine, actinomycin D, and methotrexate.},
doi = {},
url = {https://www.osti.gov/biblio/6377257}, journal = {Conn. Med.; (United States)},
number = ,
volume = 65 Suppl 3,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}