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Title: Homologous radioimmunoassay for human parathyrin (residues 53-84)

Abstract

A sequential saturation double-antibody radioimmunoassay for carboxyl-terminal fragments of human parathyrin (hPTH) in serum is described. Standards are prepared with synthetic hPTH (residues 53-84) in hPTH-free serum. Antisera are obtained by immunizing guinea pigs with partly purified hPTH extracted from adenomatous glands. Tracer is prepared by labeling hPTH (53-84), presumably at the histidine residue, with /sup 125/I by the Chloramine T method at pH 8.6. Dilution curves for hPTH extracted from adenomas are superimposable on dilution curves for the synthetic 53-84 fragment. Dilution of sera from hyperparathyroid patients showed linearity of response with concentration in the present assay, but non-linearity in the heterologous radioimmunoassay. In contrast to the heterologous system, which discriminated 28 of 32 patients with primary hyperparathyroidism from 32 normals (normal range: undetectable to 54 pmol/L, omitting the highest and lowest values from controls), the present assay separated these groups without overlap.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Heidelberg, Federal Republic of Germany
OSTI Identifier:
6377018
Resource Type:
Journal Article
Journal Name:
Clin. Chem. (Winston-Salem, N.C.); (United States)
Additional Journal Information:
Journal Volume: 28:8
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PARATHORMONE; RADIOIMMUNOASSAY; DIAGNOSIS; DISEASES; IODINE 125; PARATHYROID GLANDS; PATIENTS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Hitzler, W, Schmidt-Gayk, H, Spiropoulos, P, Raue, F, and Hufner, M. Homologous radioimmunoassay for human parathyrin (residues 53-84). United States: N. p., 1982. Web.
Hitzler, W, Schmidt-Gayk, H, Spiropoulos, P, Raue, F, & Hufner, M. Homologous radioimmunoassay for human parathyrin (residues 53-84). United States.
Hitzler, W, Schmidt-Gayk, H, Spiropoulos, P, Raue, F, and Hufner, M. 1982. "Homologous radioimmunoassay for human parathyrin (residues 53-84)". United States.
@article{osti_6377018,
title = {Homologous radioimmunoassay for human parathyrin (residues 53-84)},
author = {Hitzler, W and Schmidt-Gayk, H and Spiropoulos, P and Raue, F and Hufner, M},
abstractNote = {A sequential saturation double-antibody radioimmunoassay for carboxyl-terminal fragments of human parathyrin (hPTH) in serum is described. Standards are prepared with synthetic hPTH (residues 53-84) in hPTH-free serum. Antisera are obtained by immunizing guinea pigs with partly purified hPTH extracted from adenomatous glands. Tracer is prepared by labeling hPTH (53-84), presumably at the histidine residue, with /sup 125/I by the Chloramine T method at pH 8.6. Dilution curves for hPTH extracted from adenomas are superimposable on dilution curves for the synthetic 53-84 fragment. Dilution of sera from hyperparathyroid patients showed linearity of response with concentration in the present assay, but non-linearity in the heterologous radioimmunoassay. In contrast to the heterologous system, which discriminated 28 of 32 patients with primary hyperparathyroidism from 32 normals (normal range: undetectable to 54 pmol/L, omitting the highest and lowest values from controls), the present assay separated these groups without overlap.},
doi = {},
url = {https://www.osti.gov/biblio/6377018}, journal = {Clin. Chem. (Winston-Salem, N.C.); (United States)},
number = ,
volume = 28:8,
place = {United States},
year = {Sun Aug 01 00:00:00 EDT 1982},
month = {Sun Aug 01 00:00:00 EDT 1982}
}