Separation of DNA restriction fragments using capillary electrophoresis
- Iowa State Univ., Ames (United States)
Gel-filled and non-gel' capillary electrophoresis (CE) have been applied to the separation of various DNA restriction fragments. 30% HydroLink gel, polymerized inside a 75[mu]m i.d. fused-silica capillary, was used in the gel-filled CE. Primary results show that the HL capillary gel was simple to cast, and its stability was reasonably good under the running conditions. In the non-gel CE experiment, a buffer containing the sieving additive hydroxypropylmethyl cellulose was used to affect the size-dependent separation. The use of GC capillaries eliminates the inconvenience of separately coating the capillary walls for efficient non-gel separation. Finally, the authors demonstrate that it is feasible to detect native DNA fragments using indirect fluorometry in non-gel capillary electrophoresis.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6376666
- Journal Information:
- Journal of Liquid Chromatography; (United States), Vol. 16:9-10; ISSN 0148-3919
- Country of Publication:
- United States
- Language:
- English
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