Measurement of intact human parathyrin by an extracting two-site immunoradiometric assay

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6375405

Blind, E; Schmidt-Gayk, H; Armbruster, F P; Stadler, A

This is an immunoradiometric assay of intact human parathyrin, hPTH(1-84). One antibody, directed against the N-terminal part of the hormone, was produced in goats and conjugated covalently to cellulose particles. hPTH(1-84) and the N-terminal fragments were extracted from EDTA-treated plasma by these particles and thus concentrated. Another antibody, against synthetic hPTH(53-84), was raised in rabbits; this bound to the C-terminal part of the hormone. The final step was labeling the second free binding site of this antibody with /sup 125/I-labeled Tyr52-hPTH(53-84) and measuring the bound radioactivity. This assay can detect intact PTH in concentrations as low as 0.6 pmol/L (1.2 X 10(-16) mol per tube). The assay did not cross react with hPTH(1-34), hPTH(1-44), hPTH(28-48), hPTH(39-84), hPTH(44-68), or hPTH(53-84) in concentrations up to 6400 pmol/L. In 60 normal subjects, hPTH(1-84) concentrations ranged from 1.9 to 6.8 pmol/L; in 32 patients with primary hyperparathyroidism, from 7.0 to 80 pmol/L. The hormone was not detected in four patients with hypoparathyroidism.

• Research Organization: Chirurgische Universitaetsklinik, Heidelberg, Germany, F.R.
• OSTI ID: 6375405
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 33:8
• Country of Publication: United States
• Language: English

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550201* - Biochemistry- Tracer Techniques