Proteins cross-linked to U1 RNA by ultraviolet irradiation
Proteins associated to U1 RNA react with antinuclear antibodies obtained from sera of patients with autoimmune diseases. K.B. cells were labeled with /sup 3/H-amino acids of /sup 3/H-uridine. The U1 RNA particles were immunoprecipitated. Protein and RNA analysis was by electrophoresis and fluorography. The ratio of material at the top of the RNA gel to the U1 RNA band was 0.79. After exposure of the cells to UV irradiation, the ratio was 5.04. A substantial amount of U1 RNA was cross-linked to proteins or other nuclear components. Protein patterns obtained from U1 RNP particles subjected to UV irradiation revealed a change in electrophoretic mobility by four proteins in the molecular range of 14,000 to 28,000 daltons. The 33,000 dalton protein was reduced by 50% but its electrophoretic mobility was unchanged. The protein band at 68,000 daltons was unchanged in amount or mobility by UV irradiation. It is concluded that the low molecular weight proteins (14,000 - 28,000) and the protein of 33,000 daltons are in close proximity to U1 RNA and possibly play a role in the molecular function(s) attributed to U1 RNA.
- Research Organization:
- Univ. of Puerto Rico, San Juan
- OSTI ID:
- 6368540
- Report Number(s):
- CONF-870644-; TRN: 87-034124
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
C-reactive protein reacts with the U1 small nuclear ribonucleoprotein
Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N
Related Subjects
ANIMAL CELLS
GENETIC RADIATION EFFECTS
PROTEINS
CROSS-LINKING
RNA
AMINO ACIDS
ANTIBODIES
ELECTROPHORESIS
MOLECULAR WEIGHT
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
URIDINE
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
URACILS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture