Immunological crossreactivity between Lp(a) and the third component of complement
Lipoprotein(a) (Lp(a)) is known to be an independent risk factor for atherosclerosis, yet little is known about its physiological role. During the authors investigation of the site of synthesis of Lp(a), they made the observation that the third component of complement (C3) reacts with antisera to Lp(a). Hep G-2 cells or rhesus liver organ cultures were labelled using TVS-methionine in serum-free, methionine-free DMEM. The labelled media were immunoprecipitated with antisera raised in the goat and the rabbit against Lp(a) and apolipoprotein(a) (apo(a)). The immunoprecipitates were subjected to electrophoresis on 2.5 - 16% gradient gels and visualized by autoradiography. All antisera consistently precipitated a significant amount of C3. This reaction with C3 could be inhibited by competition with pure preparations of Lp(a) and apo(a) or by preincubation of the medium with antiserum to C3. In addition, immunoprecipitation of C3 by anti-C3 was inhibited by competition with both apo(a) and Lp(a). No cross-reactivity was observed with antisera to apoB, apoE, normal rabbit serum, or purified non-specific rabbit IgG. This unexpected crossreactivity between Lp(a) and C3, observed in vitro, may have biological relevance and suggests a possible relationship between Lp(a) and the immune system.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 6359738
- Report Number(s):
- CONF-870644-; TRN: 87-028671
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nerve injury stimulates the secretion of apolipoprotein E by nonneuronal cells
Polarized secretion of newly synthesized lipoproteins by the Caco-2 human intestinal cell line
Related Subjects
ARTERIOSCLEROSIS
PATHOGENESIS
COMPLEMENT
IMMUNE REACTIONS
LIPOPROTEINS
BIOLOGICAL FUNCTIONS
AUTORADIOGRAPHY
ELECTROPHORESIS
METHIONINE
SULFUR 35
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
FUNCTIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
VASCULAR DISEASES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques