Poly(ADP-ribosyl)ation of a herpes simplex virus immediate early polypeptide

Journal Article · Thu Dec 01 00:00:00 EST 1983 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(83)90515-9· OSTI ID:6355184

Preston, C M; Notarianni, E L

In vitro poly(ADP-ribosyl)ation of the herpes simplex virus type 1 (HSV-1) immediate early polypeptide Vmw175 is reported. The phenomenon was most clearly observed by use of the temperature-sensitive mutant tsK, which overproduces Vmw175 at the nonpermissive temperature (NPT) and has a mutation in the coding sequences for this polypeptide. Nuclei prepared from cells which were infected with tsK at NPT and subsequently downshifted to the permissive temperature incorporated (/sup 32/P)NAD into Vmw175. This reaction did not occur when nuclei were prepared from cells constantly maintained at NPT, showing that only functional Vmw175 can be radiolabeled with (/sup 32/P)NAD. The identity of the acceptor protein was confirmed by demonstrating the expected electrophoretic mobility differences between the HSV-1 and HSV-2 counterparts of Vmw175. The use of suitable inhibitors demonstrated that the reaction represented mono- or poly(ADP-ribosyl)ation, and further analysis showed the presence of long poly(ADP-ribose) chains attached to Vmw175. Poly(ADP-ribosyl)ation may be important as a cause or result of the regulation of viral transcription by Vmw175. Radiolabeling of another virus-specified polypeptide (approximate molecular weight 38,000), thought to be a structural component of the input virus, is also reported.

• Research Organization: MRC Virology Unit, Glasgow, Scotland
• OSTI ID: 6355184
• Journal Information: Virology; (United States), Vol. 131:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HERPES SIMPLEX
GENE MUTATIONS
POLYPEPTIDES
BIOCHEMICAL REACTION KINETICS
MOLECULAR STRUCTURE
ADP
IN VITRO
LABELLING
NAD
PHOSPHORUS 32
RIBOSE
TEMPERATURE DEPENDENCE
TRANSCRIPTION
ALDEHYDES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
COENZYMES
DAYS LIVING RADIOISOTOPES
DISEASES
INFECTIOUS DISEASES
ISOTOPES
KINETICS
LIGHT NUCLEI
MONOSACCHARIDES
MUTATIONS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PENTOSES
PEPTIDES
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SKIN DISEASES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques