Cell killing, radiosensitization and cell cycle redistribution induced by chronic hypoxia
Some of the biological changes associated with extreme hypoxia at 37/sup 0/C (less than 10 ppM pO/sub 2/) were examined in Chinese hamster V79 cells. Specifically, extreme hypoxia caused an initial decrease in plating efficiency to 55% in 4 hr after the onset of hypoxia. Beyond this time, the decline in plating efficiency was more gradual reaching 35% of control at 20 hr. Flow microfluorimetry (FMF) studies, in which cells are sorted on the basis of DNA content and then assayed for viability, demonstrated that mid S phase cells were most sensitive to chronic hypoxia. Hypoxia also caused alterations in the cell cycle distribution of initially asynchronous cells, as determined by dual parameter FMF measurements of both cellular DNA content and incorporated BudR. Lastly, cells stored in chronic hypoxia displayed an enhanced radiosensitivity when compared to acutely hypoxic cells.
- Research Organization:
- George Washington Univ. Medical Center, Washington, DC
- OSTI ID:
- 6354661
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 10:8
- Country of Publication:
- United States
- Language:
- English
Similar Records
Differing sensitivity to fluorescent light in Chinese hamster cells containing equally incorporated quantities of BUdR versus IUdR
Evaluation of nitroimidazole hypoxic cell radiosensitizers in a human tumor cell line high in intracellular glutathione
Related Subjects
ANOXIA
RADIOSENSITIVITY EFFECTS
CELL CYCLE
KINETICS
CELL KILLING
RADIOINDUCTION
CELL FLOW SYSTEMS
DNA
FIBROBLASTS
FLUORESCENCE
HAMSTERS
X RADIATION
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LUMINESCENCE
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)