Role of the adenylate energy charge in the response of Chinese hamster ovary cells to radiation
Steady-state modification of the adenylate energy charge in aerobic Chinese hamster ovary (CHO) cells was achieved with a combination of rotenone and 2-deoxy glucose (2dG). The radiation response of these cells was not significantly affected by this treatment when cells were irradiated either one or three hours after addition of the drugs, and held in the presence of the inhibitors for one hour after irradiation. The ability of cells to repair radiation-induced single-strand breaks was studied by the alkaline elution method. Energy depleted cells repaired single-strand breaks at a slightly slower rate than the controls. However, thymidine incorporation was also inhibited, suggesting that repair may still have preceded events leading to the fixation of that damage (e.g., DNA replication).
- Research Organization:
- Stanford Univ. Medical School, CA
- OSTI ID:
- 6354629
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 10:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ATP
RADIOSENSITIVITY EFFECTS
CELL KILLING
RADIOINDUCTION
STRAND BREAKS
CHO CELLS
DNA REPAIR
DNA REPLICATION
THYMIDINE
TRITIUM COMPOUNDS
UPTAKE
X RADIATION
ANIMAL CELLS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LABELLED COMPOUNDS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
560121* - Radiation Effects on Cells- External Source- (-1987)
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)
550301 - Cytology- Tracer Techniques