Methylation of nucleolar RNA in HeLa cells studied by autoradiography

Journal Article · Sun Jan 01 00:00:00 EST 1984 · J. Ultrastruct. Res.; (United States)

DOI:https://doi.org/10.1016/S0022-5320(84)90094-7· OSTI ID:6350145

Cervera, J; Martinez, A; Renau-Piqueras, J

Methylation of nucleolar RNA was studied by autoradiography in HeLa cells using L-(methyl-/sup 3/H)methionine and S-adenosyl-L-(methyl-/sup 3/H)methionine as radioactive precursors. Pulse-labeling experiments show that nucleolar RNA methylation occurs on the newly synthesized RNA at the nucleolar fibrillar RNP component and mostly on the fibrillar ring of fibrillar centers, where pre-rRNA is being synthesized. Pulse-chase experiments show a shift of silver grains from the nucleolar fibrillar RNP component to the nucleolar granular component first and then to the cytoplasm. Labeling of nucleolar RNA via specific methylation permits the study of intranucleolar processing of pre-rRNA and confirms the sequence of labeling of the two nucleolar RNP components observed with radioactive uridine.

• Research Organization: Instituto de Investigaciones Citologicas, Caja de Ahorros de Valencia, Spain
• OSTI ID: 6350145
• Journal Information: J. Ultrastruct. Res.; (United States), Vol. 86:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HELA CELLS
AUTORADIOGRAPHY
RNA
RADIOBIOLOGY
METHIONINE
METHYLATION
NUCLEOLI
TRITIUM COMPOUNDS
AMINO ACIDS
BIOLOGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DRUGS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
550201* - Biochemistry- Tracer Techniques