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Title: Structural analysis of the CD11b gene and phylogenetic analysis of the [alpha]-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution

Abstract

CD11b is a member of the [beta]2 subfamily of the human leukocyte integrins. Its expression is limited to mature myeloid and NK cells and is up-regulated during the course of granulocytic and monocytic differentiation. The CD11b/CD18 (Mo1) heterodimer promotes adhesion of granulocytes and monocytes to C3bi-coated bacteria and endothelial cells. In an attempt to relate the exon structure to the known functional domains, as well as to identify and study cis-acting elements that are involved in its tissue-specific expression, the authors have isolated genomic clones encoding CD11b, deduced the exon/intron organization, and determined the transcriptional start site. The CD11b gene spans 55 kb and is encoded by 30 exons. Its structure closely resembles that of CD11c, another of the three leukocyte integrin [alpha]-chains, and suggests that these two genes arose by a gene duplication event. Furthermore, comparison of the CD11b gene structure with that of platelet glycoprotein llb and Drosophila PS2 suggest how the human leukocyte integrins evolved and dispersed during the course of evolution. 67 refs., 5 figs., 2 tabs.

Authors:
; ;  [1];  [2];  [3]
  1. Beth Israel Hospital, Boston, MA (United States)
  2. Beth Israel Hospital, Boston, MA (United States) Harvard Medical School, Boston, MA (United States)
  3. Boston Univ., MA (United States)
Publication Date:
OSTI Identifier:
6337033
Resource Type:
Journal Article
Journal Name:
Journal of Immunology; (United States)
Additional Journal Information:
Journal Volume: 150:2; Journal ID: ISSN 0022-1767
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; BIOLOGICAL EVOLUTION; LEUKOCYTES; ADHESION; CELL DIFFERENTIATION; MEMBRANE PROTEINS; MONOCYTES; BACTERIA; DNA SEQUENCING; GENE REGULATION; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; MATERIALS; MICROORGANISMS; ORGANIC COMPOUNDS; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Fleming, J C, Gonzalez, D A, Tenen, D G, Pahl, H L, and Smith, T F. Structural analysis of the CD11b gene and phylogenetic analysis of the [alpha]-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution. United States: N. p., 1993. Web.
Fleming, J C, Gonzalez, D A, Tenen, D G, Pahl, H L, & Smith, T F. Structural analysis of the CD11b gene and phylogenetic analysis of the [alpha]-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution. United States.
Fleming, J C, Gonzalez, D A, Tenen, D G, Pahl, H L, and Smith, T F. 1993. "Structural analysis of the CD11b gene and phylogenetic analysis of the [alpha]-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution". United States.
@article{osti_6337033,
title = {Structural analysis of the CD11b gene and phylogenetic analysis of the [alpha]-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution},
author = {Fleming, J C and Gonzalez, D A and Tenen, D G and Pahl, H L and Smith, T F},
abstractNote = {CD11b is a member of the [beta]2 subfamily of the human leukocyte integrins. Its expression is limited to mature myeloid and NK cells and is up-regulated during the course of granulocytic and monocytic differentiation. The CD11b/CD18 (Mo1) heterodimer promotes adhesion of granulocytes and monocytes to C3bi-coated bacteria and endothelial cells. In an attempt to relate the exon structure to the known functional domains, as well as to identify and study cis-acting elements that are involved in its tissue-specific expression, the authors have isolated genomic clones encoding CD11b, deduced the exon/intron organization, and determined the transcriptional start site. The CD11b gene spans 55 kb and is encoded by 30 exons. Its structure closely resembles that of CD11c, another of the three leukocyte integrin [alpha]-chains, and suggests that these two genes arose by a gene duplication event. Furthermore, comparison of the CD11b gene structure with that of platelet glycoprotein llb and Drosophila PS2 suggest how the human leukocyte integrins evolved and dispersed during the course of evolution. 67 refs., 5 figs., 2 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6337033}, journal = {Journal of Immunology; (United States)},
issn = {0022-1767},
number = ,
volume = 150:2,
place = {United States},
year = {Fri Jan 15 00:00:00 EST 1993},
month = {Fri Jan 15 00:00:00 EST 1993}
}