Stimulation by epinephrine of the membrane transport of long chain fatty acid in the adipocyte
In isolated rat adipocytes, epinephrine rapidly stimulates the transport of long chain fatty acid across the plasma membrane. At a concentration of unbound oleate of 0.1 microM and 5 min exposure to the hormone, the minimal effective concentration of epinephrine is 0.03 and the optimal concentration 0.3 microM (0.01 and 0.1 microgram/ml). The stimulated rates are 5-10-fold the basal rate of influx or efflux. The hormone effect is on the transport process specifically as shown by isolation of the product of transport in either direction as unesterified fatty acid and inhibition by the transport inhibitors phloretin and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. This effect of epinephrine on transport coordinates physiologically with lipase activation to bring about fatty acid release from adipose tissue.
- Research Organization:
- Vanderbilt Univ. School of Medicine, Nashville, TN
- OSTI ID:
- 6335795
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADRENALINE
BIOLOGICAL EFFECTS
OLEIC ACID
MEMBRANE TRANSPORT
TRITIUM COMPOUNDS
TRACER TECHNIQUES
ADIPOSE TISSUE
IN VITRO
KINETICS
RATS
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CONNECTIVE TISSUE
DRUGS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques